Nanjing Shuangxindashi Trading Co., Ltd.

First, the raw material pre-treatment stage is initiated.High-quality protein raw materials (such as soy protein, fish collagen protein, etc.) are selected. After cleaning and grinding, they undergo low-temperature defatting and impurity removal processes to eliminate fat and coarse fibers, etc. Then, they undergo pre-activation treatment before enzymatic hydrolysis to enhance the reaction efficiency between the raw materials and the enzyme preparation, laying the foundation for the subsequent generation of peptide chains.

In the enzymatic hydrolysis reaction stage, the pre-treated raw materials are put into the enzymatic hydrolysis reactor, and specific proteases (such as trypsin, alkaline protease) are added in the prescribed proportion. Under precisely controlled conditions of temperature (35-55℃) and pH value (5.0-8.0), the proteins are hydrolyzed directionally through uniform stirring, breaking the large molecular proteins into small peptide chains. During the reaction process, the hydrolysis degree is monitored in real time. Once the preset standard is reached, the reaction is terminated by high-temperature inactivation of enzymes or adjustment of pH value to prevent excessive hydrolysis that may affect the activity of the peptides.

Then, the separation and purification stage begins. First, through plate-and-frame filtration, large-molecule impurities that have not been hydrolyzed are removed. Then, using membrane separation equipment, peptides with improper molecular weights are retained, and the target molecular weight range of peptide solutions is selected. Subsequently, with the help of the high-performance liquid chromatography preparation system, further separation and purification are carried out to remove residual impurities and free amino acids, thereby improving the purity of the peptides.

Finally, comes the concentration drying and product preparation. The purified peptide solution is concentrated to a certain concentration using a vacuum concentration equipment, and then sent to a freeze-drying machine. In a low-temperature and vacuum environment, the moisture is removed to obtain loose peptide powder. The finished product needs to undergo particle size screening, uniformity mixing, and then undergo aseptic packaging. Eventually, a standard peptide product is formed. The entire process is closely linked. From raw materials to the finished product, all key parameters are strictly controlled throughout the process to ensure uniform product quality, safety and reliability.